dxshIgG HRP Aff Pur (1 mL)-抗绵羊-二抗&对照-优宁维抗体平台
dxshIgG HRP Aff Pur (1 mL)
货号: HAF016 品牌: R&D Systems 同种亚型:
IgG
IgG
反应种属:
Sheep
Sheep
应用:
Western Blot, Simple Western
Western Blot, Simple Western
规格: 1mL
产品说明书
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价格: ¥ 1664.00

目录价: ¥1664.00

数量
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概述
别名
immunoglobulin heavy constant gamma 1 (G1m marker)
全称
Immunoglobulin G
同种亚型
IgG
反应种属
Sheep
应用
Western Blot, Simple Western
形式
Sodium Phosphate and Proclin 300.
目标/特异性
Detects sheep IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with mouse IgG, rabbit IgG, human IgG, and chicken IgY is observed.
使用方法
Western Blot: Use at a 1:1000 dilution.
Simple Western: Use at a 1:50 dilution.
是否允许进口
允许
是否危险品
非危险品
性能
供应商
R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Evidence Suggesting a Role of Iron in a Mouse Model of Nephrogenic Systemic Fibrosis.
Authors: Bose C, Megyesi J, Shah S, Hiatt K, Hall K, Karaduta O, Swaminathan S
PLoS ONE, 2015;10(8):e0136563.
Species: Human
Sample Type: Cell Lysates
Application: WB
A Novel Locus Harbouring a Functional CD164 Nonsense Mutation Identified in a Large Danish Family with Nonsyndromic Hearing Impairment.
Authors: Nyegaard M, Rendtorff N, Nielsen M, Corydon T, Demontis D, Starnawska A, Hedemand A, Buniello A, Niola F, Overgaard M, Leal S, Ahmad W, Wikman F, Petersen K, Cruger D, Oostrik J, Kremer H, Tommerup N, Frodin M, Steel K, Tranebjaerg L, Borglum A
PLoS Genet, 2015;11(7):e1005386.
Species: Human
Sample Type: Cell Lysates

A Cre-conditional MYCN-driven neuroblastoma mouse model as an improved tool for preclinical studies.
Authors: Althoff K, Beckers A, Bell E, Nortmeyer M, Thor T, Sprussel A, Lindner S, De Preter K, Florin A, Heukamp L, Klein-Hitpass L, Astrahantseff K, Kumps C, Speleman F, Eggert A, Westermann F, Schramm A, Schulte J
Oncogene, 2015;34(26):3357-68.
Species: Human
Sample Type: Tissue Homogenates
Application: WB
Bacterial delivery of Staphylococcus aureus alpha-hemolysin causes regression and necrosis in murine tumors.
Authors: St Jean A, Swofford C, Panteli J, Brentzel Z, Forbes N
Mol Ther, 2014;22(7):1266-74.
Species: Bacteria - E. Coli
Sample Type: Cell Lysates
Application: WB
Structure-function analysis of Nel, a thrombospondin-1-like glycoprotein involved in neural development and functions.
Authors: Nakamura R, Nakamoto C, Obama H
J. Biol. Chem., 2012;287(5):3282-91.
Species: Sheep
Sample Type: IgG
Application: WB
Nkx3.2 promotes primary chondrogenic differentiation by upregulating Col2a1 transcription.
Authors: Kawato Y, Hirao M, Ebina K, Shi K, Hashimoto J, Honjo Y, Yoshikawa H, Myoui A
PLoS ONE, 2012;7(4):e34703.
Species: Mouse
Sample Type: Cell Lysates
Application: WB
Neurocan contributes to the molecular heterogeneity of the perinodal ECM.
Authors: Bekku Y, Oohashi T,
Arch. Histol. Cytol., 2010;73(2):95-102.
Species: Mouse
Sample Type: Tissue Homogenates

纯化方式
Antigen Affinity-purified
免疫原
Sheep IgG
生物活性
Sheep
标记(有特殊处理)
背景

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

存放说明
4 degrees C
参考文献
  • New Tools: NorthernLights New Fluorescent Secondary Antibodies

参考图片

Detection of SheepIgG Primary Antibody by Western Blot Western blot shows detection of Sheep Anti-Human/Mouse/Rat PSMA1Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF7565)using a 1:1000 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody(Catalog # HAF016).  This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.

Detection of Sheep IgG Primary Antibody by Simple WesternTM. Simple Western lane view shows detection of Sheep Anti-Mouse Contactin-6 Polyclonal Antibody
(Catalog #AF5890) using a 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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