rhEGF, CF (1 mg)-细胞因子-2D/3D培养-优宁维细胞/细胞培养平台
rhEGF, CF (1 mg)
货号: 236-EG-01M 品牌: R&D Systems 反应种属:
Human
Human
规格: 1mg
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价格: ¥ 5530.00

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概述
别名
beta-urogastrone; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone
全称
Epidermal Growth Factor
反应种属
Human
形式
Lyophilized from a 0.2 μm filtered solution in PBS.
是否允许进口
允许
是否危险品
非危险品
性能
供应商
R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Transcription factor-mediated regulation of kidney versus liver specification of Slc and Abc drug transporters, tight junction molecules and signature biomarkers
Authors: G Martovetsk, KT Bush, SK Nigam
Drug Metab Dispos, 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Application of single-cell RNA sequencing in optimizing a combinatorial therapeutic strategy in metastatic renal cell carcinoma
Genome Biol, 2016;17(1):80.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Pituitary Adenlylate Cyclase Activating Peptide Protects Adult Neural Stem Cells from a Hypoglycaemic milieu
Authors: Shiva Mansouri
PLoS ONE, 2016;11(6):e0156867.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Treatment of Triple-Negative Breast Cancer with TORC1/2 Inhibitors Sustains a Drug-Resistant and Notch-Dependent Cancer Stem Cell Population
Authors: Neil E Bhola
Cancer Res, 2016;76(2):440-52.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Cancer stem cell-secreted macrophage migration inhibitory factor stimulates myeloid derived suppressor cell function and facilitates glioblastoma immune evasion
Stem Cells, 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
The polarity protein Scribble positions DLC3 at adherens junctions to regulate Rho signaling
J Cell Sci, 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
SPINK6 promotes metastasis of nasopharyngeal carcinoma via binding and activation of epithelial growth factor receptor
Cancer Res, 2016;0(0):.
Species: Human
Sample Type: recombinant protein
Application: Bioassay
Glucocorticoids Suppress Mitochondrial Oxidant Production via Upregulation of Uncoupling Protein 2 in Hyperglycemic Endothelial Cells
PLoS ONE, 2016;11(4):e0154813.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
LINC00520 is induced by Src, STAT3, and PI3K and plays a functional role in breast cancer
Oncotarget, 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Growth hormone is permissive for neoplastic colon growth
Proc Natl Acad Sci USA, 2016;0(0):.
Species: Human
Sample Type: Whole Tissue
Application: Bioassay
Acetylation of the RhoA GEF Net1A controls its subcellular localization and activity.
Authors: Song E, Oh W, Ulu A, Carr H, Zuo Y, Frost J
J Cell Sci, 2015;128(5):913-22.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Early intervention for spinal cord injury with human induced pluripotent stem cells oligodendrocyte progenitors.
Authors: All A, Gharibani P, Gupta S, Bazley F, Pashai N, Chou B, Shah S, Resar L, Cheng L, Gearhart J, Kerr C
PLoS ONE, 2015;10(1):e0116933.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Mitochondrial control by DRP1 in brain tumor initiating cells.
Authors: Xie Q, Wu Q, Horbinski C, Flavahan W, Yang K, Zhou W, Dombrowski S, Huang Z, Fang X, Shi Y, Ferguson A, Kashatus D, Bao S, Rich J
Nat Neurosci, 2015;18(4):501-10.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
EphrinB2 controls vessel pruning through STAT1-JNK3 signalling.
Authors: Salvucci O, Ohnuki H, Maric D, Hou X, Li X, Yoon S, Segarra M, Eberhart C, Acker-Palmer A, Tosato G
Nat Commun, 2015;6(0):6576.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Endothelial-mesenchymal transition of brain endothelial cells: possible role during metastatic extravasation.
Authors: Krizbai I, Gasparics A, Nagyoszi P, Fazakas C, Molnar J, Wilhelm I, Bencs R, Rosivall L, Sebe A
PLoS ONE, 2015;10(3):e0119655.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Epigenetic regulation of Elf5 is associated with epithelial-mesenchymal transition in urothelial cancer.
Authors: Wu, Bo, Cao, Xiaoming, Liang, Xuezhi, Zhang, Xuhui, Zhang, Wei, Sun, Guang, Wang, Dongwen
PLoS ONE, 2015;10(1):e0117510.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
High levels of KAP1 expression are associated with aggressive clinical features in ovarian cancer.
Authors: Cui Y, Yang S, Fu X, Feng J, Xu S, Ying G
Int J Mol Sci, 2015;16(1):363-77.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Let-7 Sensitizes KRAS Mutant Tumor Cells to Chemotherapy.
Authors: Dai X, Jiang Y, Tan C
PLoS ONE, 2015;10(5):e0126653.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Persistence and toxin production by Clostridium difficile within human intestinal organoids result in disruption of epithelial paracellular barrier function.
Authors: Leslie J, Huang S, Opp J, Nagy M, Kobayashi M, Young V, Spence J
Infect Immun, 2015;83(1):138-45.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Upregulated WDR5 promotes proliferation, self-renewal and chemoresistance in bladder cancer via mediating H3K4 trimethylation.
Authors: Chen X, Xie W, Gu P, Cai Q, Wang B, Xie Y, Dong W, He W, Zhong G, Lin T, Huang J
Sci Rep, 2015;5(0):8293.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
The evidence of glioblastoma heterogeneity.
Authors: Soeda, Akio, Hara, Akira, Kunisada, Takahiro, Yoshimura, Shin-ich, Iwama, Toru, Park, Deric M
Sci Rep, 2015;5(0):7979.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Activin/nodal signaling switches the terminal fate of human embryonic stem cell-derived trophoblasts.
Authors: Sarkar P, Randall S, Collier T, Nero A, Russell T, Muddiman D, Rao B
J Biol Chem, 2015;290(14):8834-48.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Arf6 regulates EGF-induced internalization of E-cadherin in breast cancer cells.
Authors: Xu R, Zhang Y, Gu L, Zheng J, Cui J, Dong J, Du J
Cancer Cell Int, 2015;15(1):11.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Natural killer (NK) cells inhibit systemic metastasis of glioblastoma cells and have therapeutic effects against glioblastomas in the brain.
Authors: Lee S, Kang W, Yoon Y, Jin J, Song H, Her J, Kang S, Hwang Y, Kang K, Joo K, Nam D
BMC Cancer, 2015;15(1):1011.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
RAP1-mediated MEK/ERK pathway defects in Kabuki syndrome.
Authors: Bogershausen N, Tsai I, Pohl E, Kiper P, Beleggia F, Percin E, Keupp K, Matchan A, Milz E, Alanay Y, Kayserili H, Liu Y, Banka S, Kranz A, Zenker M, Wieczorek D, Elcioglu N, Prontera P, Lyonnet S, Meitinger T, Stewart A, Donnai D, Strom T, Boduroglu K, Yigit G, Li Y, Katsanis N, Wollnik B
J Clin Invest, 2015;125(9):3585-99.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
The Synergistic Local Immunosuppressive Effects of Neural Stem Cells Expressing Indoleamine 2,3-Dioxygenase (IDO) in an Experimental Autoimmune Encephalomyelitis (EAE) Animal Model.
Authors: Lee Y, An J, Lee K, Kim S, Song H, Pyeon H, Nam H, Kang K, Joo K
PLoS ONE, 2015;10(12):e0144298.
Species: Rat
Sample Type: Whole Cells
Application: Bioassay
mTOR Directs Breast Morphogenesis through the PKC-alpha-Rac1 Signaling Axis.
Authors: Morrison M, Young C, Wang S, Sobolik T, Sanchez V, Hicks D, Cook R, Brantley-Sieders D
PLoS Genet, 2015;11(7):e1005291.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Excision of Expanded GAA Repeats Alleviates the Molecular Phenotype of Friedreich's Ataxia.
Authors: Li Y, Polak U, Bhalla A, Rozwadowska N, Butler J, Lynch D, Dent S, Napierala M
Mol Ther, 2015;23(6):1055-65.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Functional genomic analysis identifies indoxyl sulfate as a major, poorly dialyzable uremic toxin in end-stage renal disease.
Authors: Jhawar S, Singh P, Torres D, Ramirez-Valle F, Kassem H, Banerjee T, Dolgalev I, Heguy A, Zavadil J, Lowenstein J
PLoS ONE, 2015;10(3):e0118703.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Two Isoforms of the RNA Binding Protein, Coding Region Determinant-binding Protein (CRD-BP/IGF2BP1), Are Expressed in Breast Epithelium and Support Clonogenic Growth of Breast Tumor Cells.
Authors: Fakhraldeen S, Clark R, Roopra A, Chin E, Huang W, Castorino J, Wisinski K, Kim T, Spiegelman V, Alexander C
J Biol Chem, 2015;290(21):13386-400.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Epigenetic Activation of TWIST1 by MTDH Promotes Cancer Stem-like Cell Traits in Breast Cancer.
Authors: Liang Y, Hu J, Li J, Liu Y, Yu J, Zhuang X, Mu L, Kong X, Hong D, Yang Q, Hu G
Cancer Res, 2015;75(17):3672-80.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Characterisation of mesothelioma-initiating cells and their susceptibility to anti-cancer agents.
Authors: Pasdar E, Smits M, Stapelberg M, Bajzikova M, Stantic M, Goodwin J, Yan B, Stursa J, Kovarova J, Sachaphibulkij K, Bezawork-Geleta A, Sobol M, Filimonenko A, Tomasetti M, Zobalova R, Hozak P, Dong L, Neuzil J
PLoS ONE, 2015;10(5):e0119549.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Mutation of androgen receptor N-terminal phosphorylation site Tyr-267 leads to inhibition of nuclear translocation and DNA binding.
Authors: Karaca M, Liu Y, Zhang Z, De Silva D, Parker J, Earp H, Whang Y
PLoS ONE, 2015;10(5):e0126270.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Pan-HER, an Antibody Mixture Simultaneously Targeting EGFR, HER2, and HER3, Effectively Overcomes Tumor Heterogeneity and Plasticity.
Authors: Jacobsen H, Poulsen T, Dahlman A, Kjaer I, Koefoed K, Sen J, Weilguny D, Bjerregaard B, Andersen C, Horak I, Pedersen M, Kragh M, Lantto J
Clin Cancer Res, 2015;21(18):4110-22.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Selective calcium sensitivity in immature glioma cancer stem cells.
Authors: Wee S, Niklasson M, Marinescu V, Segerman A, Schmidt L, Hermansson A, Dirks P, Forsberg-Nilsson K, Westermark B, Uhrbom L, Linnarsson S, Nelander S, Andang M
PLoS ONE, 2014;9(12):e115698.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Transcriptional profiling of adult neural stem-like cells from the human brain.
Authors: Sandberg C, Vik-Mo E, Behnan J, Helseth E, Langmoen I
PLoS ONE, 2014;9(12):e114739.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Treatment of acute respiratory distress syndrome with allogeneic adipose-derived mesenchymal stem cells: a randomized, placebo-controlled pilot study.
Authors: Zheng G, Huang L, Tong H, Shu Q, Hu Y, Ge M, Deng K, Zhang L, Zou B, Cheng B, Xu J
Respir Res, 2014;15(0):39.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
An in vitro cord formation assay identifies unique vascular phenotypes associated with angiogenic growth factors.
Authors: Falcon B, Swearingen M, Gough W, Lee L, Foreman R, Uhlik M, Hanson J, Lee J, McClure D, Chintharlapalli S
PLoS ONE, 2014;9(9):e106901.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
EGF potentiation of VEGF production is cell density dependent in H292 EGFR wild type NSCLC cell line.
Authors: Ranayhossaini D, Lu J, Mabus J, Gervais A, Lingham R, Fursov N
Int J Mol Sci, 2014;15(10):17686-704.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Rho GTPases RhoA and Rac1 mediate effects of dietary folate on metastatic potential of A549 cancer cells through the control of cofilin phosphorylation.
Authors: Oleinik N, Helke K, Kistner-Griffin E, Krupenko N, Krupenko S
J Biol Chem, 2014;289(38):26383-94.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Chromatinized protein kinase C-theta directly regulates inducible genes in epithelial to mesenchymal transition and breast cancer stem cells.
Authors: Zafar A, Wu F, Hardy K, Li J, Tu W, McCuaig R, Harris J, Khanna K, Attema J, Gregory P, Goodall G, Harrington K, Dahlstrom J, Boulding T, Madden R, Tan A, Milburn P, Rao S
Mol Cell Biol, 2014;34(16):2961-80.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Additive effects of EGF and IL-1beta regulate tumor cell migration and invasion in gastric adenocarcinoma via activation of ERK1/2.
Authors: Han J, Xie Y, Lan F, Yu Y, Liu W, Chen J, Zheng F, Ouyang X, Lin X, Lin Y, Huang Q, Wang L, Tan J
Int J Oncol, 2014;45(1):291-301.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Nestin regulates proliferation, migration, invasion and stemness of lung adenocarcinoma.
Authors: Narita K, Matsuda Y, Seike M, Naito Z, Gemma A, Ishiwata T
Int J Oncol, 2014;44(4):1118-30.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Glioma cancer stem cells secrete Gremlin1 to promote their maintenance within the tumor hierarchy.
Authors: Yan K, Wu Q, Yan D, Lee C, Rahim N, Tritschler I, DeVecchio J, Kalady M, Hjelmeland A, Rich J
Genes Dev, 2014;28(10):1085-100.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Sulforaphane, quercetin and catechins complement each other in elimination of advanced pancreatic cancer by miR-let-7 induction and K-ras inhibition.
Authors: Appari M, Babu K, Kaczorowski A, Gross W, Herr I
Int J Oncol, 2014;45(4):1391-400.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Sulforaphane and TRAIL induce a synergistic elimination of advanced prostate cancer stem-like cells.
Authors: Labsch S, Liu L, Bauer N, Zhang Y, Aleksandrowicz E, Gladkich J, Schonsiegel F, Herr I
Int J Oncol, 2014;44(5):1470-80.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
The broad-spectrum metalloproteinase inhibitor BB-94 inhibits growth, HER3 and Erk activation in fulvestrant-resistant breast cancer cell lines.
Authors: Kirkegaard T, Yde C, Kveiborg M, Lykkesfeldt A
Int J Oncol, 2014;45(1):393-400.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Triptolide reverses hypoxia-induced epithelial-mesenchymal transition and stem-like features in pancreatic cancer by NF-kappaB downregulation.
Authors: Liu L, Salnikov A, Bauer N, Aleksandrowicz E, Labsch S, Nwaeburu C, Mattern J, Gladkich J, Schemmer P, Werner J, Herr I
Int J Cancer, 2014;134(10):2489-503.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Multiple receptor tyrosine kinases converge on microRNA-134 to control KRAS, STAT5B, and glioblastoma.
Authors: Zhang, Y, Kim, J, Mueller, A C, Dey, B, Yang, Y, Lee, D-h, Hachmann, J, Finderle, S, Park, D M, Christensen, J, Schiff, D, Purow, B, Dutta, A, Abounader, R
Cell Death Differ, 2014;21(5):720-34.
Species: Human
Sample Type: whole cells
Application: Bioassay
TGF-beta1 promotes lymphangiogenesis during peritoneal fibrosis.
Authors: Kinashi H, Ito Y, Mizuno M, Suzuki Y, Terabayashi T, Nagura F, Hattori R, Matsukawa Y, Mizuno T, Noda Y, Nishimura H, Nishio R, Maruyama S, Imai E, Matsuo S, Takei Y
J Am Soc Nephrol, 2013;24(10):1627-42.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Targeting protein kinase CK2 suppresses prosurvival signaling pathways and growth of glioblastoma.
Authors: Zheng Y, McFarland B, Drygin D, Yu H, Bellis S, Kim H, Bredel M, Benveniste E
Clin Cancer Res, 2013;19(23):6484-94.
Species: Human
Sample Type: Whole Cells
Application: cell culture
Vaccination for invasive canine meningioma induces in situ production of antibodies capable of antibody-dependent cell-mediated cytotoxicity.
Authors: Andersen B, Pluhar G, Seiler C, Goulart M, SantaCruz K, Schutten M, Meints J, O'Sullivan M, Bentley R, Packer R, Thomovsky S, Chen A, Faissler D, Chen W, Hunt M, Olin M, Ohlfest J
Cancer Res, 2013;73(10):2987-97.
Species: Canine
Sample Type: Whole Cells
Application: Bioassay
Insulin signaling via Akt2 switches plakophilin 1 function from stabilizing cell adhesion to promoting cell proliferation.
Authors: Wolf A, Rietscher K, Glass M, Huttelmaier S, Schutkowski M, Ihling C, Sinz A, Wingenfeld A, Mun A, Hatzfeld M
J Cell Sci, 2013;126(0):1832-44.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Enhanced filopodium formation and stem-like phenotypes in a novel metastatic head and neck cancer cell model.
Authors: Chen Y, Huang W, Chang S, Chang K, Kao S, Lo J, Su P
Oncol Rep, 2013;30(6):2829-37.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Inhibition of PARP1 by small interfering RNA enhances docetaxel activity against human prostate cancer PC3 cells.
Authors: Wu W, Kong Z, Duan X, Zhu H, Li S, Zeng S, Liang Y, Iliakis G, Gui Z, Yang D
Biochem Biophys Res Commun, 2013;442(1):127-32.
Species: Human
Sample Type: whole cells
Application: cell culture
mTOR-dependent proliferation defect in human ES-derived neural stem cells affected by myotonic dystrophy type 1.
Authors: Denis J, Gauthier M, Rachdi L, Aubert S, Giraud-Triboult K, Poydenot P, Benchoua A, Champon B, Maury Y, Baldeschi C, Scharfmann R, Pietu G, Peschanski M, Martinat C
J Cell Sci, 2013;126(0):1763-72.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Chemotherapy activates cancer-associated fibroblasts to maintain colorectal cancer-initiating cells by IL-17A.
Authors: Lotti, Fiorenza, Jarrar, Awad M, Pai, Rish K, Hitomi, Masahiro, Lathia, Justin, Mace, Adam, Gantt, Gerald A, Sukhdeo, Kumar, DeVecchio, Jennifer, Vasanji, Amit, Leahy, Patrick, Hjelmeland, Anita B, Kalady, Matthew, Rich, Jeremy N
J Exp Med, 2013;210(13):2851-72.
Species: Human
Sample Type: whole cells
Application: Bioassay
Diacylglycerol kinase delta modulates Akt phosphorylation through pleckstrin homology domain leucine-rich repeat protein phosphatase 2 (PHLPP2).
Authors: Crotty T, Nakano T, Stafforini D, Topham M
J Biol Chem, 2013;288(3):1439-47.
Species: Human
Sample Type: whole cells
Application: Bioassay
The novel c-Met inhibitor cabozantinib overcomes gemcitabine resistance and stem cell signaling in pancreatic cancer.
Authors: Hage C, Rausch V, Giese N, Giese T, Schonsiegel F, Labsch S, Nwaeburu C, Mattern J, Gladkich J, Herr I
Cell Death Dis, 2013;4(0):e627.
Species: Human
Sample Type: whole cells
Application: Bioassay
Distinct effects of ligand-induced PDGFRalpha and PDGFRbeta signaling in the human rhabdomyosarcoma tumor cell and stroma cell compartments.
Authors: Ehnman M, Missiaglia E, Folestad E, Selfe J, Strell C, Thway K, Brodin B, Pietras K, Shipley J, Ostman A, Eriksson U
Cancer Res, 2013;73(7):2139-49.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
The MAPK pathway is a predominant regulator of HLA-A expression in esophageal and gastric cancer.
Authors: Mimura, Kousaku, Shiraishi, Kensuke, Mueller, Anja, Izawa, Shinichi, Kua, Ley-Fang, So, Jimmy, Yong, Wei-Peng, Fujii, Hideki, Seliger, Barbara, Kiessling, Rolf, Kono, Koji
J Immunol, 2013;191(12):6261-72.
Species: Human
Sample Type: Whole Cells
Application: cell culture
Oncogenic K-Ras and loss of Smad4 mediate invasion by activating an EGFR/NF-kappaB Axis that induces expression of MMP9 and uPA in human pancreas progenitor cells.
Authors: Bera A, Zhao S, Cao L, Chiao P, Freeman J
PLoS ONE, 2013;8(12):e82282.
Species: Human
Sample Type: whole cells
Application: Bioassay
Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c.
Authors: Di Maggio N, Mehrkens A, Papadimitropoulos A, Schaeren S, Heberer M, Banfi A, Martin I
Stem Cells, 2012;30(7):1455-64.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Both LRP5 and LRP6 receptors are required to respond to physiological Wnt ligands in mammary epithelial cells and fibroblasts.
Authors: Goel S, Chin EN, Fakhraldeen SA
J. Biol. Chem., 2012;287(20):16454-66.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Activation of HER family signaling as a mechanism of acquired resistance to ALK inhibitors in EML4-ALK-positive non-small cell lung cancer.
Authors: Tanizaki J, Okamoto I, Okabe T, Sakai K, Tanaka K, Hayashi H, Kaneda H, Takezawa K, Kuwata K, Yamaguchi H, Hatashita E, Nishio K, Nakagawa K
Clin Cancer Res, 2012;18(22):6219-26.
Species: Human
Sample Type: whole cells
Application: Bioassay
The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1.
Authors: Zelinka C, Scott M, Volkov L, Fischer A
PLoS ONE, 2012;7(9):e44477.
Species: Chicken
Sample Type: In Vivo
Application: In Vivo
Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
Authors: Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM
Stem Cells Dev., 2012;21(3):404-10.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Regulation of IL-33 expression by IFN-gamma and tumor necrosis factor-alpha in normal human epidermal keratinocytes.
J. Invest. Dermatol., 2012;132(11):2593-600.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012;487(7408):505-9.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Vandetanib inhibits both VEGFR-2 and EGFR signalling at clinically relevant drug levels in preclinical models of human cancer.
Authors: Brave SR, Odedra R, James NH
Int. J. Oncol., 2011;39(1):271-8.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro.
Authors: Spence JR, Mayhew CN, Rankin SA
Nature, 2011;470(7332):105-9.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
CD133 positive embryonal rhabdomyosarcoma stem-like cell population is enriched in rhabdospheres.
Authors: Walter D, Satheesha S, Albrecht P, Bornhauser BC, D'Alessandro V, Oesch SM, Rehrauer H, Leuschner I, Koscielniak E, Gengler C, Moch H, Bernasconi M, Niggli FK, Schafer BW
PLoS ONE, 2011;6(5):e19506.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Kallikrein expression and cathelicidin processing are independently controlled in keratinocytes by calcium, vitamin D(3), and retinoic acid.
Authors: Morizane S, Yamasaki K, Kabigting FD, Gallo RL
J. Invest. Dermatol., 2010;130(5):1297-306.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Bmi1 is essential in Twist1-induced epithelial-mesenchymal transition.
Authors: Yang MH, Hsu DS, Wang HW
Nat. Cell Biol., 2010;12(10):982-92.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
The chitinase-like protein YKL-40 is secreted by airway epithelial cells at base line and in response to compressive mechanical stress.
Authors: Park JA, Drazen JM, Tschumperlin DJ
J. Biol. Chem., 2010;285(39):29817-25.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
AZD8931, an equipotent, reversible inhibitor of signaling by epidermal growth factor receptor, ERBB2 (HER2), and ERBB3: a unique agent for simultaneous ERBB receptor blockade in cancer.
Authors: Hickinson DM, Klinowska T, Speake G
Clin. Cancer Res., 2010;16(4):1159-69.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Notch and Wnt signaling mediated rod photoreceptor regeneration by Muller cells in adult mammalian retina.
Authors: Del Debbio CB, Balasubramanian S, Parameswaran S, Chaudhuri A, Qiu F, Ahmad I
PLoS ONE, 2010;5(8):e12425.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Multiple receptor tyrosine kinases regulate HIF-1alpha and HIF-2alpha in normoxia and hypoxia in neuroblastoma: implications for antiangiogenic mechanisms of multikinase inhibitors.
Authors: Nilsson MB, Zage PE, Zeng L, Xu L, Cascone T, Wu HK, Saigal B, Zweidler-McKay PA, Heymach JV
Oncogene, 2010;29(20):2938-49.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Endocytosis of flotillin-1 and flotillin-2 is regulated by Fyn kinase.
Authors: Riento K, Frick M, Schafer I, Nichols BJ
J. Cell. Sci., 2009;122(0):912-8.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Docosahexaenoic acid promotes neuronal differentiation by regulating basic helix-loop-helix transcription factors and cell cycle in neural stem cells.
Authors: Katakura M, Hashimoto M, Shahdat HM, Gamoh S, Okui T, Matsuzaki K, Shido O
Neuroscience, 2009;160(3):651-60.
Species: Rat
Sample Type: Whole Cells
Application: Bioassay
Enhanced radiosensitivity and radiation-induced apoptosis in glioma CD133-positive cells by knockdown of SirT1 expression.
Authors: Chang CJ, Hsu CC, Yung MC, Chen KY, Tzao C, Wu WF, Chou HY, Lee YY, Lu KH, Chiou SH, Ma HI
Biochem. Biophys. Res. Commun., 2009;380(2):236-42.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Dithiolethione compounds inhibit Akt signaling in human breast and lung cancer cells by increasing PP2A activity.
Authors: Switzer CH, Ridnour LA, Cheng RY, Sparatore A, Del Soldato P, Moody TW, Vitek MP, Roberts DD, Wink DA
Oncogene, 2009;28(43):3837-46.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Mitogen-activated protein kinase-signaling regulates the ability of Muller glia to proliferate and protect retinal neurons against excitotoxicity.
Authors: Fischer AJ, Scott MA, Ritchey ER, Sherwood P
Glia, 2009;57(14):1538-52.
Species: Chicken
Sample Type: In Vivo
Application: In Vivo
Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis.
Authors: Lee HS, Myers A, Kim J
Am. J. Respir. Crit. Care Med., 2009;180(11):1056-67.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
EphrinA5 acts as a tumor suppressor in glioma by negative regulation of epidermal growth factor receptor.
Authors: Li JJ, Liu DP, Liu GT, Xie D
Oncogene, 2009;28(15):1759-68.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Purification and characterization of mouse lacrimal gland epithelial cells and reconstruction of an acinarlike structure in three-dimensional culture.
Authors: Ueda Y, Karasawa Y, Satoh Y, Nishikawa S, Imaki J, Ito M
Invest. Ophthalmol. Vis. Sci., 2009;50(5):1978-87.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Epidermal Growth Factor Receptor Blockade in Combination with Conventional Chemotherapy Inhibits Soft Tissue Sarcoma Cell Growth In vitro and In vivo.
Authors: Ren W, Korchin B, Zhu QS, Wei C, Dicker A, Heymach J, Lazar A, Pollock RE, Lev D
Clin. Cancer Res., 2008;14(9):2785-2795.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Activation of the epidermal growth factor receptor (EGFR) by a novel metalloprotease pathway.
Authors: Bergin DA, Greene CM, Sterchi EE, Kenna C, Geraghty P, Belaaouaj A, Taggart CC, O'Neill SJ, McElvaney NG
J. Biol. Chem., 2008;283(46):31736-44.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Human epidermal growth factor receptor (HER-1:HER-3) Fc-mediated heterodimer has broad antiproliferative activity in vitro and in human tumor xenografts.
Authors: Sarup J, Jin P, Turin L, Bai X, Beryt M, Brdlik C, Higaki JN, Jorgensen B, Lau FW, Lindley P, Liu J, Ni I, Rozzelle J, Kumari R, Watson SA, Zhang J, Shepard HM
Mol. Cancer Ther., 2008;7(10):3223-36.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Lineage selection of functional and cryopreservable human embryonic stem cell-derived neurons.
Authors: Ladewig J, Koch P, Endl E, Meiners B, Opitz T, Couillard-Despres S, Aigner L, Brustle O
Stem Cells, 2008;26(7):1705-12.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Characterization of human skin-derived mesenchymal stem cell proliferation rate in different growth conditions.
Authors: Riekstina U, Muceniece R, Cakstina I, Muiznieks I, Ancans J
Cytotechnology, 2008;58(3):153-62.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage.
Authors: Elkabetz Y, Panagiotakos G, Al Shamy G, Socci ND, Tabar V, Studer L
Genes Dev., 2008;22(2):152-65.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Thalidomide inhibits epidermal growth factor-induced cell growth in mouse and human monocytic leukemia cells via Ras inactivation.
Authors: Noman AS, Koide N, Khuda II, Dagvadorj J, Tumurkhuu G, Naiki Y, Komatsu T, Yoshida T, Yokochi T
Biochem. Biophys. Res. Commun., 2008;374(4):683-7.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Long-term maintenance of brain tumor stem cell properties under at non-adherent and adherent culture conditions.
Authors: Inagaki A, Soeda A, Oka N, Kitajima H, Nakagawa J, Motohashi T, Kunisada T, Iwama T
Biochem. Biophys. Res. Commun., 2007;361(3):586-92.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
The effects of IL-20 subfamily cytokines on reconstituted human epidermis suggest potential roles in cutaneous innate defense and pathogenic adaptive immunity in psoriasis.
Authors: Sa SM, Valdez PA, Wu J
J. Immunol., 2007;178(4):2229-40.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Atrial-radiofrequency catheter ablation mediated targeting of mesenchymal stromal cells.
Authors: Schweizer PA, Krause U, Becker R, Seckinger A, Bauer A, Hardt C, Eckstein V, Ho AD, Koenen M, Katus HA, Zehelein J
Stem Cells, 2007;25(6):1546-51.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Growth factor induction of Cripto-1 shedding by glycosylphosphatidylinositol-phospholipase D and enhancement of endothelial cell migration.
Authors: Watanabe K, Bianco C, Strizzi L, Hamada S, Mancino M, Bailly V, Mo W, Wen D, Miatkowski K, Gonzales M, Sanicola M, Seno M, Salomon DS
J. Biol. Chem., 2007;282(43):31643-55.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Tissue engineering for full-thickness burns: a dermal substitute from bench to bedside.
Authors: Kellouche S, Martin C, Korb G, Rezzonico R, Bouard D, Benbunan M, Dubertret L, Soler C, Legrand C, Dosquet C
Biochem. Biophys. Res. Commun., 2007;363(3):472-8.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
EGF-like ligands stimulate osteoclastogenesis by regulating expression of osteoclast regulatory factors by osteoblasts: implications for osteolytic bone metastases.
Authors: Zhu J, Jia X, Xiao G, Kang Y, Partridge NC, Qin L
J. Biol. Chem., 2007;282(37):26656-64.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells.
Authors: Wolff GS, Chiang PJ, Smith SM, Romero R, Armant DR
Biol. Reprod., 2007;77(1):53-60.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Epidermal growth factor receptor expression regulates proliferation in the postnatal rat retina.
Authors: Close JL, Liu J, Gumuscu B, Reh TA
Glia, 2006;54(2):94-104.
Species: Rat
Sample Type: In Vivo
Application: In Vivo
Targeting ADAM-mediated ligand cleavage to inhibit HER3 and EGFR pathways in non-small cell lung cancer.
Authors: Zhou BB, Peyton M, He B, Liu C, Girard L, Caudler E, Lo Y, Baribaud F, Mikami I, Reguart N, Yang G, Li Y, Yao W, Vaddi K, Gazdar AF, Friedman SM, Jablons DM, Newton RC, Fridman JS, Minna JD, Scherle PA
Cancer Cell, 2006;10(1):39-50.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor.
Authors: Armant DR, Kilburn BA, Petkova A, Edwin SS, Duniec-Dmuchowski ZM, Edwards HJ, Romero R, Leach RE
Development, 2006;133(4):751-9.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Intracerebral infusion of glial cell line-derived neurotrophic factor promotes striatal neurogenesis after stroke in adult rats.
Authors: Kobayashi T, Ahlenius H, Thored P, Kobayashi R, Kokaia Z, Lindvall O
Stroke, 2006;37(9):2361-7.
Species: Rat
Sample Type: Whole Cells
Application: Bioassay
Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
Authors: Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK
Blood, 2006;108(3):928-35.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines.
Authors: Rhodes KE, Raivich G, Fawcett JW
Neuroscience, 2006;140(1):87-100.
Species: Rat
Sample Type: In Vivo
Application: In Vivo
Retinal neurons regulate proliferation of postnatal progenitors and Muller glia in the rat retina via TGF beta signaling.
Authors: Close JL, Gumuscu B, Reh TA
Development, 2005;132(13):3015-26.
Species: Rat
Sample Type: In Vivo
Application: In Vivo
Effect of cytokine treatment on the neurogenesis process in the brain of soman-poisoned mice.
Authors: Collombet JM, Four E, Burckhart MF, Masqueliez C, Bernabe D, Baubichon D, Herodin F, Lallement G
Toxicology, 2005;210(1):9-23.
Species: Mouse
Sample Type: In Vivo
Application: In Vivo
Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype.
Authors: Leach RE, Kilburn B, Wang J, Liu Z, Romero R, Armant DR
Dev. Biol., 2004;266(2):223-37.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Interferon-gamma inhibits transforming growth factor-beta production in human airway epithelial cells by targeting Smads.
Authors: Wen FQ, Liu X, Kobayashi T, Abe S, Fang Q, Kohyama T, Ertl R, Terasaki Y, Manouilova L, Rennard SI
Am. J. Respir. Cell Mol. Biol., 2004;30(6):816-22.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Different aspects of gliosis in retinal Muller glia can be induced by CNTF, insulin, and FGF2 in the absence of damage.
Authors: Fischer AJ, Omar G, Eubanks J, McGuire CR, Dierks BD, Reh TA
Mol. Vis., 2004;10(0):973-86.
Species: Chicken
Sample Type: In Vivo
Application: In Vivo
Human bronchial epithelial cells express and secrete MMP-12.
Authors: Lavigne MC, Thakker P, Gunn J, Miyashiro JS, Wasserman AM, Wei SQ, Pelker JW
Biochem. Biophys. Res. Commun., 2004;324(2):534-46.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Wa5 is a novel ENU-induced antimorphic allele of the epidermal growth factor receptor.
Authors: Lee D, Cross SH, Strunk KE, Morgan JE, Bailey CL, Jackson IJ, Threadgill DW
Mamm. Genome, 2004;15(7):525-36.
Species: Mouse
Sample Type: In Vivo
Application: In Vivo
High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells.
Authors: Ni HT, Hu S, Sheng WS, Olson JM, Cheeran MC, Chan AS, Lokensgard JR, Peterson PK
Brain Res. Dev. Brain Res., 2004;152(2):159-69.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
Blood, 2004;104(2):314-20.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Wnt regulation of progenitor maturation in the cortex depends on Shh or fibroblast growth factor 2.
Authors: Viti J, Gulacsi A, Lillien L
J. Neurosci., 2003;23(13):5919-27.
Species: Mouse
Sample Type: Whole Tissue
Application: Bioassay
Dual roles of IL-4 in lung injury and fibrosis.
Authors: Huaux F, Liu T, McGarry B, Ullenbruch M, Phan SH
J. Immunol., 2003;170(4):2083-92.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Metabolic replacement of kidney function in uremic animals with a bioartificial kidney containing human cells.
Authors: Humes HD, Fissell WH, Weitzel WF, Buffington DA, Westover AJ, MacKay SM, Gutierrez JM
Am. J. Kidney Dis., 2002;39(5):1078-87.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
RGD-peptides for tissue engineering of articular cartilage.
Authors: Jeschke B, Meyer J, Jonczyk A, Kessler H, Adamietz P, Meenen NM, Kantlehner M, Goepfert C, Nies B
Biomaterials, 2002;23(16):3455-63.
Species: Porcine
Sample Type: Whole Cells
Application: Bioassay
A profibrotic function of IL-12p40 in experimental pulmonary fibrosis.
Authors: Huaux F, Arras M, Tomasi D, Barbarin V, Delos M, Coutelier JP, Vink A, Phan SH, Renauld JC, Lison D
J. Immunol., 2002;169(5):2653-61.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Apical and basolateral EGF receptors regulate gastric mucosal paracellular permeability.
Authors: Chen MC, Goliger J, Bunnett N, Soll AH
Am. J. Physiol. Gastrointest. Liver Physiol., 2001;280(2):G264-72.
Species: Canine
Sample Type: Whole Cells
Application: Bioassay
Regulation of vascular endothelial growth factor expression in human colon cancer by insulin-like growth factor-I.
Authors: Akagi Y, Liu W, Zebrowski B, Xie K, Ellis LM
Cancer Res., 1998;58(17):4008-14.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Protein kinase A can block EphA2 receptor-mediated cell repulsion by increasing EphA2 S897 phosphorylation.
Authors: Barquilla A, Lamberto I, Heynen-Genel S, Brill L, Pasquale E
Mol Biol Cell, 0;27(17):2757-70.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
alpha-Taxilin interacts with sorting nexin 4 and participates in the recycling pathway of transferrin receptor.
Authors: Sakane H, Horii Y, Nogami S, Kawano Y, Kaneko-Kawano T, Shirataki H
PLoS ONE, 0;9(4):e93509.
Species: Human
Sample Type: Whole Cells
Application: cell culture

内毒素水平
<0.10 EU per 1 μg of the protein by the LAL method.
生物活性
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is typically 20-100 pg/mL.
The specific activity of Recombinant Human EGF is approximately 1.4 x 103 IU/μg, which is calibrated against human EGF WHO International Standard (NIBSC code: 91/530).
溶解方法
Reconstitute at 500 μg/mL in sterile PBS.
背景
Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin‑binding EGF‑like growth factor (HB‑EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of the EGF family share a structural motif, the EGF‑like domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues (2). All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The 1207 amino acid (aa) human EGF precursor contains nine EGF domains and nine LDLR class B repeats. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region (3). Mature human EGF shares 70% aa sequence identity with mature mouse and rat EGF. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). These receptors undergo a complex pattern of ligand induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.
SDS-PAGE
6 kDa, reducing conditions
存放说明
Frost Free Freezer -20 degrees C
纯度
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
参考文献
  1. Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.
  2. Carpenter, G. and Cohen, S. (1990) J. Biol. Chem. 265:7709.
  3. Bell, G.I. et al. (1986) Nucl. Acids Res. 14:8427.
  4. Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res. 164:1.
  5. Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.
  6. Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.
  7. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.
  8. Qian, X. et al. (1999) J. Biol. Chem. 274:574.

参考图片

1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE  and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.

Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is typically 20‑100 pg/mL.

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