rhWnt-3a (10 ug)-细胞因子-2D/3D培养-优宁维细胞/细胞培养平台
rhWnt-3a (10 ug)
货号: 5036-WN-010 品牌: R&D Systems 反应种属:
Human
Human
规格: 10ug
产品说明书
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价格: ¥ 4583.00

数量
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概述
别名
MGC119418; MGC119419; MGC119420; protein Wnt-3a; wingless-type MMTV integration site family, member 3A
全称
Wingless-type MMTV Integration Site Family, Member 3a
反应种属
Human
形式
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
是否允许进口
允许
是否危险品
非危险品
性能
供应商
R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

The AKAP GSKIP regulates beta-catenin through both its interactions with PKA and GSK3beta
J Biol Chem, 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
TNIK inhibition abrogates colorectal cancer stemness
Nat Commun, 2016;7(0):12586.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Anthelmintic niclosamide disrupts the interplay of p65 and FOXM1/?-catenin and eradicates leukemia stem cells in chronic myelogenous leukemia
Clin Cancer Res, 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Activation of endothelial ?-catenin signaling induces heart failure
Sci Rep, 2016;6(0):25009.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Growth hormone is permissive for neoplastic colon growth
Proc Natl Acad Sci USA, 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Epsin is required for Dishevelled stability and Wnt signalling activation in colon cancer development.
Authors: Chang B, Tessneer K, McManus J, Liu X, Hahn S, Pasula S, Wu H, Song H, Chen Y, Cai X, Dong Y, Brophy M, Rahman R, Ma J, Xia L, Chen H
Nat Commun, 2015;6(0):6380.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
A Regulatory Network Involving beta-Catenin, e-Cadherin, PI3k/Akt, and Slug Balances Self-Renewal and Differentiation of Human Pluripotent Stem Cells In Response to Wnt Signaling.
Authors: Huang T, Li L, Moalim-Nour L, Jia D, Bai J, Yao Z, Bennett S, Figeys D, Wang L
Stem Cells, 2015;33(5):1419-33.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells.
Authors: Huang S, Green M, de Carvalho A, Mumau M, Chen Y, D'Souza S, Snoeck H
Nat Protoc, 2015;10(3):413-25.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Activin-A and Bmp4 levels modulate cell type specification during CHIR-induced cardiomyogenesis.
Authors: Kim M, Horst A, Blinka S, Stamm K, Mahnke D, Schuman J, Gundry R, Tomita-Mitchell A, Lough J
PLoS ONE, 2015;10(2):e0118670.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Robust derivation of epicardium and its differentiated smooth muscle cell progeny from human pluripotent stem cells.
Authors: Iyer D, Gambardella L, Bernard W, Serrano F, Mascetti V, Pedersen R, Talasila A, Sinha S
Development, 2015;142(8):1528-41.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Novel cancer chemotherapy hits by molecular topology: dual Akt and Beta-catenin inhibitors.
Authors: Zanni R, Galvez-Llompart M, Morell C, Rodriguez-Henche N, Diaz-Laviada I, Recio-Iglesias M, Garcia-Domenech R, Galvez J
PLoS ONE, 2015;10(4):e0124244.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Intestinal Commitment and Maturation of Human Pluripotent Stem Cells Is Independent of Exogenous FGF4 and R-spondin1.
Authors: Tamminen K, Balboa D, Toivonen S, Pakarinen M, Wiener Z, Alitalo K, Otonkoski T
PLoS ONE, 2015;10(7):e0134551.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells.
Authors: Wong, Amy P, Chin, Stephani, Xia, Sunny, Garner, Jodi, Bear, Christin, Rossant, Janet
Nat Protoc, 2015;10(3):363-81.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Characterization and propagation of tumor initiating cells derived from colorectal liver metastases: trials, tribulations and a cautionary note.
Authors: James M, Howells L, Karmokar A, Higgins J, Greaves P, Cai H, Dennison A, Metcalfe M, Garcea G, Lloyd D, Berry D, Steward W, Brown K
PLoS ONE, 2015;10(2):e0117776.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Endogenously produced Indian Hedgehog regulates TGFbeta-driven chondrogenesis of human bone marrow stromal/stem cells.
Authors: Handorf A, Chamberlain C, Li W
Stem Cells Dev, 2015;24(8):995-1007.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Canonical Wnt pathway inhibitor ICG-001 induces cytotoxicity of multiple myeloma cells in Wnt-independent manner.
Authors: Grigson E, Ozerova M, Pisklakova A, Liu H, Sullivan D, Nefedova Y
PLoS ONE, 2015;10(1):e0117693.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Focal adhesion kinase and Wnt signaling regulate human ductal carcinoma in situ stem cell activity and response to radiotherapy.
Authors: Williams K, Bundred N, Landberg G, Clarke R, Farnie G
Stem Cells, 2015;33(2):327-41.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Transcriptome analysis of wnt3a-treated triple-negative breast cancer cells.
Authors: Maubant S, Tesson B, Maire V, Ye M, Rigaill G, Gentien D, Cruzalegui F, Tucker G, Roman-Roman S, Dubois T
PLoS ONE, 2015;10(4):e0122333.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
An investigation of the effects of the core protein telomerase reverse transcriptase on Wnt signaling in breast cancer cells.
Authors: Listerman I, Gazzaniga F, Blackburn E
Mol Cell Biol, 2014;34(2):280-9.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Brivanib attenuates hepatic fibrosis in vivo and stellate cell activation in vitro by inhibition of FGF, VEGF and PDGF signaling.
Authors: Nakamura I, Zakharia K, Banini B, Mikhail D, Kim T, Yang J, Moser C, Shaleh H, Thornburgh S, Walters I, Roberts L
PLoS ONE, 2014;9(4):e92273.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Luminal microbes promote monocyte-stem cell interactions across a healthy colonic epithelium.
Authors: Skoczek D, Walczysko P, Horn N, Parris A, Clare S, Williams M, Sobolewski A
J Immunol, 2014;193(1):439-51.
Species: Human
Sample Type: Whole Tissue
Application: Bioassay
Zrf1 is required to establish and maintain neural progenitor identity.
Authors: Aloia L, Di Stefano B, Sessa A, Morey L, Santanach A, Gutierrez A, Cozzuto L, Benitah S, Graf T, Broccoli V, Di Croce L
Genes Dev, 2014;28(2):182-97.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Wnt/beta-catenin signaling regulates the proliferation and differentiation of mesenchymal progenitor cells through the p53 pathway.
Authors: Peng X, Yang L, Chang H, Dai G, Wang F, Duan X, Guo L, Zhang Y, Chen G
PLoS ONE, 2014;9(5):e97283.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
The metastasis suppressor NDRG1 modulates the phosphorylation and nuclear translocation of beta-catenin through mechanisms involving FRAT1 and PAK4.
Authors: Jin R, Liu W, Menezes S, Yue F, Zheng M, Kovacevic Z, Richardson D
J Cell Sci, 2014;127(0):3116-30.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Tissue-engineered vascular grafts created from human induced pluripotent stem cells.
Authors: Sundaram S, One J, Siewert J, Teodosescu S, Zhao L, Dimitrievska S, Qian H, Huang A, Niklason L
Stem Cells Transl Med, 2014;3(12):1535-43.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Glycogen synthase kinase 3 inhibitors induce the canonical WNT/beta-catenin pathway to suppress growth and self-renewal in embryonal rhabdomyosarcoma.
Authors: Chen E, DeRan M, Ignatius M, Grandinetti K, Clagg R, McCarthy K, Lobbardi R, Brockmann J, Keller C, Wu X, Langenau D
Proc Natl Acad Sci U S A, 2014;111(14):5349-54.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Drugging a stem cell compartment using Wnt3a protein as a therapeutic.
Authors: Dhamdhere, Girija R, Fang, Mark Y, Jiang, Jie, Lee, Katherin, Cheng, Du, Olveda, Rebecca, Liu, Bo, Mulligan, Kimberle, Carlson, Jeffery, Ransom, Ryan C, Weis, William, Helms, Jill A
PLoS ONE, 2014;9(1):e83650.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells.
Authors: Ekstrom E, Bergenfelz C, von Bulow V, Serifler F, Carlemalm E, Jonsson G, Andersson T, Leandersson K
Mol Cancer, 2014;13(0):88.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Cripto-1 enhances the canonical Wnt/β-catenin signaling pathway by binding to LRP5 and LRP6 co-receptors.
Cell Signal, 2013;25(1):178-89.
Species: Human
Sample Type: whole cells
Application: Bioassay
IQGAP1 protein regulates nuclear localization of beta-catenin via importin-beta5 protein in Wnt signaling.
Authors: Goto T, Sato A, Adachi S, Iemura S, Natsume T, Shibuya H
J Biol Chem, 2013;288(51):36351-60.
Species: Human
Sample Type: cells
Application: cell culture
Intrapatient variations in type 1 diabetes-specific iPS cell differentiation into insulin-producing cells.
Authors: Thatava T, Kudva Y, Edukulla R, Squillace K, De Lamo J, Khan Y, Sakuma T, Ohmine S, Terzic A, Ikeda Y
Mol Ther, 2013;21(1):228-39.
Species: Human
Sample Type: whole cells
Application: Bioassay
Atypical protein kinase Ciota is required for Wnt3a-dependent neurite outgrowth and binds to phosphorylated dishevelled 2.
Authors: Greer Y, Fields A, Brown A, Rubin J
J Biol Chem, 2013;288(13):9438-46.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Enrichment of human embryonic stem cell-derived NKX6.1-expressing pancreatic progenitor cells accelerates the maturation of insulin-secreting cells in vivo.
Authors: Rezania A, Bruin J, Xu J, Narayan K, Fox J, O'Neil J, Kieffer T
Stem Cells, 2013;31(11):2432-42.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
The Wnt signalling pathway is upregulated in an in vitro model of acquired tamoxifen resistant breast cancer.
Authors: Loh Y, Hedditch E, Baker L, Jary E, Ward R, Ford C
BMC Cancer, 2013;13(0):174.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
The hedgehog system in ovarian follicles of cattle selected for twin ovulations and births: evidence of a link between the IGF and hedgehog systems.
Authors: Aad, Pauline, Echternkamp, Sherrill, Sypherd, David D, Schreiber, Nicole B, Spicer, Leon J
Biol Reprod, 2012;87(4):79.
Species: Bovine
Sample Type: Whole Cells
Application: Bioassay
The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells.
Authors: Ichii, Michiko, Frank, Mark Bar, Iozzo, Renato V, Kincade, Paul W
Blood, 2012;119(7):1683-92.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Derivation of cerebellar neurons from human pluripotent stem cells.
Authors: Erceg S, Lukovic D, Moreno-Manzano V, Stojkovic M, Bhattacharya S
Curr Protoc Stem Cell Biol, 2012;20(0):1H.51.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
FGF signaling via MAPK is required early and improves Activin A-induced definitive endoderm formation from human embryonic stem cells.
Authors: Sui L, Mfopou J, Geens M, Sermon K, Bouwens L
Biochem Biophys Res Commun, 2012;426(3):380-5.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Wnt5a and Wnt11 are essential for second heart field progenitor development.
Authors: Cohen E, Miller M, Wang Z, Moon R, Morrisey E
Development, 2012;139(11):1931-40.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells.
Authors: Prabakar K, Dominguez-Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, Inverardi L
Cell Transplant, 2012;21(6):1321-39.
Species: Human
Sample Type: whole cells
Application: cell culture
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012;487(7408):505-9.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro.
Authors: Spence JR, Mayhew CN, Rankin SA
Nature, 2011;470(7332):105-9.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
High levels of beta-catenin signaling reduce osteogenic differentiation of stem cells in inflammatory microenvironments through inhibition of the noncanonical Wnt pathway.
Authors: Liu N, Shi S, Deng M, Tang L, Zhang G, Liu N, Ding B, Liu W, Liu Y, Shi H, Liu L, Jin Y
J. Bone Miner. Res., 2011;26(9):2082-95.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
NANOG induction of fetal liver kinase-1 (FLK1) transcription regulates endothelial cell proliferation and angiogenesis.
Authors: Kohler EE, Cowan CE, Chatterjee I
Blood, 2011;117(5):1761-9.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Evidence for altered Wnt signaling in psoriatic skin.
Authors: Gudjonsson JE, Johnston A, Stoll SW
J. Invest. Dermatol., 2010;130(7):1849-59.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Canonical Wnts function as potent regulators of osteogenesis by human mesenchymal stem cells.
Authors: Liu G, Vijayakumar S, Grumolato L, Arroyave R, Qiao H, Akiri G, Aaronson SA
J. Cell Biol., 2009;185(1):67-75.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Targeting the ROR1 and ROR2 receptors in epithelial ovarian cancer inhibits cell migration and invasion.
Authors: Henry C, Llamosas E, Knipprath-Meszaros A, Schoetzau A, Obermann E, Fuenfschilling M, Caduff R, Fink D, Hacker N, Ward R, Heinzelmann-Schwarz V, Ford C
Oncotarget, 0;6(37):40310-26.
Species: Human
Sample Type: Whole Cells
Application: Bioassay

内毒素水平
<1.0 EU per 1 μg of the protein by the LAL method.
生物活性
Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is typically 5-25 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is typically <500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line.
Optimal concentrations should be determined by each laboratory for each application.
溶解方法
Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
背景

Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1). Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (2). As intracellular beta -catenin levels rise, beta -catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes (3). Endo-IWR 1 (Catalog # 3532, # PSM1324) is a cell-permeant small molecule inhibitor of Axin turnover that suppresses Wnt signal transduction by stabilizing the beta -catenin destruction complex (4). Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Wnt-3a has two N-linked glycosylation sites (Asn 87, Asn 298), and Ser 209 is modified with palmitoleic acid (6). Glycosylation and acylation are essential for efficient Wnt secretion and biological activity, respectively (6, 7). Human Wnt-3a shares 96% amino acid (aa) identity with mouse mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt3. During embryonic development, Wnt-3a is necessary for proper development of the hippocampus, anterior-posterior patterning, somite development, and tailbud formation (9-12). Wnt-3a also promotes self-renewal of hematopoietic stem cells, neural stem cells, and embryonic stem cells (13, 14).

SDS-PAGE
40 kDa, reducing conditions
存放说明
Frost Free Freezer -20 degrees C
纯度
>75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
不含载体
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

参考文献
  1. Willert, K. and Nusse, R. (2012) Cold Spring Harb. Perspect. Biol. 4:a007864.
  2. MacDonald, B.T. and X. He (2012) Cold Spring Harb. Perspect. Biol. 4:a007880.
  3. Korinek, V. et al. (1997) Science 275:1784.
  4. Chen, B. et al. (2009) Nat. Chem. Biol. 5:100.
  5. Smolich, B.D. et al. (1993) Mol. Biol. Cell 4:1267.
  6. Takada, R. et al. (2006) Dev. Cell 11:791.
  7. Komekado, H. (2007) Genes Cells 12:521.
  8. Dunty Jr. W. C. et al. (2008) Development 135:85.
  9. Ikeya, M. and S. Takada (2001) Mech. Dev. 103:27.
  10. Lee, S. M. et al. (2000) Development 127:457.
  11. Takada, S. et al. (1994) Genes Dev. 8:174.
  12. Willert, K. et al. (2003) Nature 423:6938.
  13. Kalani, M.Y. et al. (2008) Proc. Natl. Acad. Sci. USA 105:16970.
  14. Ten Berge, D. et al. (2011) Nat. Cell Biol. 13:1070.
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