rhFGF basic (146 a (500 UG)-细胞因子-2D/3D培养-优宁维细胞/细胞培养平台
rhFGF basic (146 a (500 UG)
货号: 233-FB-500 品牌: R&D Systems
规格: 500UG
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价格: ¥ 13777.00

目录价: ¥13777.00

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概述
别名
basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGF2AS; FGFBprostatropin; fibroblast growth factor 2 (basic); GFG1; HBGF-2; HBGH-2; heparin-binding growth factor 2; NUDT6; Prostatropin
全称
Fibroblast Growth Factor basic
形式
Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl with BSA as a carrier protein.
是否允许进口
允许
是否危险品
非危险品
性能
供应商
R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Endothelial heparan sulfate 6-o-sulfation levels regulate angiogenic responses of endothelial cells to fibroblast growth factor 2 and vascular endothelial growth factor.
Authors: Ferreras, Cristina, Rushton, Graham, Cole, Claire L, Babur, Muhammad, Telfer, Brian A, van Kuppevelt, Toin H, Gardiner, John M, Williams, Kaye J, Jayson, Gordon C, Avizienyte, Egle
J Biol Chem, 2102;287(43):36132-46.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Engineering the haemogenic niche mitigates endogenous inhibitory signals and controls pluripotent stem cell-derived blood emergence
Authors: N Rahman, PM Brauer, L Ho, T Usenko, M Tewary, JC Zúñiga-Pfl, PW Zandstra
Nat Commun, 2017;8(0):15380.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Brother of the regulator of the imprinted site (BORIS) variant subfamily 6 is a novel target of lung cancer stem-like cell immunotherapy
Authors: R Horibe, Y Hirohashi, T Asano, T Mariya, T Suzuki, A Takaya, H Saijo, Y Shionoya, T Kubo, M Nakatsugaw, T Kanaseki, T Tsukahara, K Watanabe, E Atsuyama, S Toji, H Hirano, T Hasegawa, H Takahashi, N Sato, T Torigoe
PLoS ONE, 2017;12(3):e0171460.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-Related Macular Degeneration
Authors: JS Saini, B Corneo, JD Miller, TR Kiehl, Q Wang, NC Boles, TA Blenkinsop, JH Stern, S Temple
Cell Stem Cell, 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Inhibition of Proliferation and Epithelial Mesenchymal Transition in Retinal Pigment Epithelial Cells by Heavy Chain-Hyaluronan/Pentraxin 3
Authors: H He, AE Kuriyan, CW Su, M Mahabole, Y Zhang, YT Zhu, HW Flynn, JM Parel, SC Tseng
Sci Rep, 2017;7(0):43736.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Targeting BMI1(+) Cancer Stem Cells Overcomes Chemoresistance and Inhibits Metastases in Squamous Cell Carcinoma
Authors: D Chen, M Wu, Y Li, I Chang, Q Yuan, M Ekimyan-Sa, P Deng, B Yu, Y Yu, J Dong, JM Szymanski, S Ramadoss, J Li, CY Wang
Cell Stem Cell, 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Atypical PKC and Notch Inhibition Differentially Modulate Cortical Interneuron Subclass Fate from Embryonic Stem Cells
Authors: DJ Tischfield, J Kim, SA Anderson
Stem Cell Reports, 2017;0(0):.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
An FAK-YAP-mTOR Signaling Axis Regulates Stem Cell-Based Tissue Renewal in Mice
Authors: JK Hu, W Du, SJ Shelton, MC Oldham, CM DiPersio, OD Klein
Cell Stem Cell, 2017;0(0):.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Ccl5 establishes an autocrine high-grade glioma growth regulatory circuit critical for mesenchymal glioblastoma survival
Authors: Y Pan, LJ Smithson, Y Ma, D Hambardzum, DH Gutmann
Oncotarget, 2017;0(0):.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Gelatin device for the delivery of growth factors involved in endochondral ossification
Authors: LA Ahrens, D Vonwil, J Christense, VP Shastri
PLoS ONE, 2017;12(4):e0175095.
Species: Chicken
Sample Type: Whole Embryo
Application: Bioassay
Large, Diverse Population Cohorts of hiPSCs and Derived Hepatocyte-like Cells Reveal Functional Genetic Variation at Blood Lipid-Associated Loci
Authors: EE Pashos, Y Park, X Wang, A Raghavan, W Yang, D Abbey, DT Peters, J Arbelaez, M Hernandez, N Kuperwasse, W Li, Z Lian, Y Liu, W Lv, SL Lytle-Gabb, DH Marchadier, P Rogov, J Shi, KJ Slovik, IM Stylianou, L Wang, R Yan, X Zhang, S Kathiresan, SA Duncan, TS Mikkelsen, EE Morrisey, DJ Rader, CD Brown, K Musunuru
Cell Stem Cell, 2017;20(4):558-570.e10.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling
Authors: KB McCauley, F Hawkins, M Serra, DC Thomas, A Jacob, DN Kotton
Cell Stem Cell, 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells
Authors: F Sampazioti, MC de Brito, I Geti, A Bertero, NR Hannan, L Vallier
Nat Protoc, 2017;12(4):814-827.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
In Vitro Micropatterned Human Pluripotent Stem Cell Test (�P-hPST) for Morphometric-Based Teratogen Screening
Authors: J Xing, Y Cao, Y Yu, H Li, Z Song, H Yu
Sci Rep, 2017;7(1):8491.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Human Pluripotent Stem Cell-Derived Atrial and Ventricular Cardiomyocytes Develop from Distinct Mesoderm Populations
Authors: JH Lee, SI Protze, Z Laksman, PH Backx, GM Keller
Cell Stem Cell, 2017;21(2):179-194.e4.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells
Authors: KG Wong, SD Ryan, K Ramnarine, SA Rosen, SE Mann, A Kulick, E De Stanchi, FJ Müller, TJ Kacmarczyk, C Zhang, D Betel, MJ Tomishima
Stem Cell Reports, 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Dnmt3a-mediated inhibition of Wnt in cardiac progenitor cells improves differentiation and remote remodeling after infarction
Authors: A De Pauw, E Andre, B Sekkali, C Bouzin, H Esfahani, N Barbier, A Loriot, C De Smet, L Vanhoutte, S Moniotte, B Gerber, V di Mauro, D Catalucci, O Feron, D Hilfiker-K, JL Balligand
JCI Insight, 2017;2(12):.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Stage-Specific Human Induced Pluripotent Stem Cells Map the Progression of Myeloid Transformation to Transplantable Leukemia
Authors: AG Kotini, CJ Chang, A Chow, H Yuan, TC Ho, T Wang, S Vora, A Solovyov, C Husser, M Olszewska, J Teruya-Fel, D Perumal, VM Klimek, A Spyridonid, RK Rampal, L Silverman, EP Reddy, E Papaemmanu, S Parekh, BD Greenbaum, CS Leslie, MG Kharas, EP Papapetrou
Cell Stem Cell, 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
The Semaphorin 4D-RhoA-Akt Signal Cascade Regulates Enamel Matrix Secretion in Coordination with Cell Polarization During Ameloblast Differentiation
J Bone Miner Res, 2016;0(0):.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Engulfed cadherin fingers are polarized junctional structures between collectively migrating endothelial�cells
Nat. Cell Biol., 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Fibroblast growth factor (FGF) signaling regulates transforming growth factor beta (TGF?)-dependent smooth muscle cell phenotype modulation
Sci Rep, 2016;6(0):33407.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Capturing the biology of disease severity in a PSC-based model of familial dysautonomia
Authors: N Zeltner, F Fattahi, NC Dubois, N Saurat, F Lafaille, L Shang, B Zimmer, J Tchieu, MA Soliman, G Lee, JL Casanova, L Studer
Nat. Med., 2016;22(12):1421-1427.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Robust derivation of epicardium and its differentiated smooth muscle cell progeny from human pluripotent stem cells.
Authors: Iyer D, Gambardella L, Bernard W, Serrano F, Mascetti V, Pedersen R, Talasila A, Sinha S
Development, 2015;142(8):1528-41.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Nerve growth factor and proNGF simultaneously promote symmetric self-renewal, quiescence, and epithelial to mesenchymal transition to enlarge the breast cancer stem cell compartment.
Authors: Tomellini E, Touil Y, Lagadec C, Julien S, Ostyn P, Ziental-Gelus N, Meignan S, Lengrand J, Adriaenssens E, Polakowska R, Le Bourhis X
Stem Cells, 2015;33(2):342-53.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
The sirtuin-2 inhibitor AK7 is neuroprotective in models of Parkinson's disease but not amyotrophic lateral sclerosis and cerebral ischemia.
Authors: Chen X, Wales P, Quinti L, Zuo F, Moniot S, Herisson F, Rauf N, Wang H, Silverman R, Ayata C, Maxwell M, Steegborn C, Schwarzschild M, Outeiro T, Kazantsev A
PLoS ONE, 2015;10(1):e0116919.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain.
Authors: Deverman B, Pravdo P, Simpson B, Kumar S, Chan K, Banerjee A, Wu W, Yang B, Huber N, Pasca S, Gradinaru V
Nat Biotechnol, 2015;34(2):204-9.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Prolonged and tunable residence time using reversible covalent kinase inhibitors.
Authors: Bradshaw J, McFarland J, Paavilainen V, Bisconte A, Tam D, Phan V, Romanov S, Finkle D, Shu J, Patel V, Ton T, Li X, Loughhead D, Nunn P, Karr D, Gerritsen M, Funk J, Owens T, Verner E, Brameld K, Hill R, Goldstein D, Taunton J
Nat Chem Biol, 2015;11(7):525-31.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
mTOR inhibition improves fibroblast growth factor receptor targeting in hepatocellular carcinoma.
Authors: Scheller, T, Hellerbrand, C, Moser, C, Schmidt, K, Kroemer, A, Brunner, S M, Schlitt, H J, Geissler, E K, Lang, S A
Br J Cancer, 2015;112(5):841-50.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
The schizophrenia risk gene product miR-137 alters presynaptic plasticity.
Authors: Siegert S, Seo J, Kwon E, Rudenko A, Cho S, Wang W, Flood Z, Martorell A, Ericsson M, Mungenast A, Tsai L
Nat Neurosci, 2015;18(7):1008-16.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Cellular Responses Modulated by FGF-2 Adsorbed on Albumin/Heparin Layer-by-Layer Assemblies.
Authors: Kumorek M, Kubies D, Filova E, Houska M, Kasoju N, Mazl Chanova E, Matejka R, Kryslova M, Bacakova L, Rypacek F
PLoS ONE, 2015;10(5):e0125484.
Species: Bovine
Sample Type: Whole Cells
Application: Bioassay
EphrinB2 controls vessel pruning through STAT1-JNK3 signalling.
Authors: Salvucci O, Ohnuki H, Maric D, Hou X, Li X, Yoon S, Segarra M, Eberhart C, Acker-Palmer A, Tosato G
Nat Commun, 2015;6(0):6576.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Improved neurological outcome by intramuscular injection of human amniotic fluid derived stem cells in a muscle denervation model.
Authors: Chen C, Cheng F, Su H, Sheu M, Lu Z, Chiang C, Yang D, Sheehan J, Pan H
PLoS ONE, 2015;10(5):e0124624.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Enhanced SMAD1 Signaling Contributes to Impairments of Early Development in CFC-iPSCs.
Authors: Han K, Kim S, Kim D, Choi J, Im I, Hwang K, Kim C, Lee B, Yoo H, Han Y
Stem Cells, 2015;33(5):1447-55.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Mitochondrial control by DRP1 in brain tumor initiating cells.
Authors: Xie Q, Wu Q, Horbinski C, Flavahan W, Yang K, Zhou W, Dombrowski S, Huang Z, Fang X, Shi Y, Ferguson A, Kashatus D, Bao S, Rich J
Nat Neurosci, 2015;18(4):501-10.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Temporal and embryonic lineage-dependent regulation of human vascular SMC development by NOTCH3.
Authors: Granata A, Bernard W, Zhao N, McCafferty J, Lilly B, Sinha S
Stem Cells Dev, 2015;24(7):846-56.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
The evidence of glioblastoma heterogeneity.
Authors: Soeda, Akio, Hara, Akira, Kunisada, Takahiro, Yoshimura, Shin-ich, Iwama, Toru, Park, Deric M
Sci Rep, 2015;5(0):7979.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Epigenetic regulation of Elf5 is associated with epithelial-mesenchymal transition in urothelial cancer.
Authors: Wu, Bo, Cao, Xiaoming, Liang, Xuezhi, Zhang, Xuhui, Zhang, Wei, Sun, Guang, Wang, Dongwen
PLoS ONE, 2015;10(1):e0117510.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Early intervention for spinal cord injury with human induced pluripotent stem cells oligodendrocyte progenitors.
Authors: All A, Gharibani P, Gupta S, Bazley F, Pashai N, Chou B, Shah S, Resar L, Cheng L, Gearhart J, Kerr C
PLoS ONE, 2015;10(1):e0116933.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Endophilin marks and controls a clathrin-independent endocytic pathway.
Authors: Boucrot E, Ferreira A, Almeida-Souza L, Debard S, Vallis Y, Howard G, Bertot L, Sauvonnet N, McMahon H
Nature, 2015;517(7535):460-5.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Histone Chaperone HIRA in Regulation of Transcription Factor RUNX1.
Authors: Majumder A, Syed K, Joseph S, Scambler P, Dutta D
J Biol Chem, 2015;290(21):13053-63.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Reciprocal cellular cross-talk within the tumor microenvironment promotes oncolytic virus activity.
Authors: Ilkow C, Marguerie M, Batenchuk C, Mayer J, Ben Neriah D, Cousineau S, Falls T, Jennings V, Boileau M, Bellamy D, Bastin D, de Souza C, Alkayyal A, Zhang J, Le Boeuf F, Arulanandam R, Stubbert L, Sampath P, Thorne S, Paramanthan P, Chatterjee A, Strieter R, Burdick M, Addison C, Stojdl D, Atkins H, Auer R, Diallo J, Lichty B, Bell J
Nat Med, 2015;21(5):530-6.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Resveratrol ameliorates the maturation process of beta-cell-like cells obtained from an optimized differentiation protocol of human embryonic stem cells.
Authors: Pezzolla D, Lopez-Beas J, Lachaud C, Dominguez-Rodriguez A, Smani T, Hmadcha A, Soria B
PLoS ONE, 2015;10(3):e0119904.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells.
Authors: Huang S, Green M, de Carvalho A, Mumau M, Chen Y, D'Souza S, Snoeck H
Nat Protoc, 2015;10(3):413-25.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Glioma cancer stem cells secrete Gremlin1 to promote their maintenance within the tumor hierarchy.
Authors: Yan K, Wu Q, Yan D, Lee C, Rahim N, Tritschler I, DeVecchio J, Kalady M, Hjelmeland A, Rich J
Genes Dev, 2014;28(10):1085-100.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa.
Authors: Tolar J, McGrath J, Xia L, Riddle M, Lees C, Eide C, Keene D, Liu L, Osborn M, Lund T, Blazar B, Wagner J
J Invest Dermatol, 2014;134(5):1246-54.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Selective calcium sensitivity in immature glioma cancer stem cells.
Authors: Wee S, Niklasson M, Marinescu V, Segerman A, Schmidt L, Hermansson A, Dirks P, Forsberg-Nilsson K, Westermark B, Uhrbom L, Linnarsson S, Nelander S, Andang M
PLoS ONE, 2014;9(12):e115698.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Adult human nasal mesenchymal-like stem cells restore cochlear spiral ganglion neurons after experimental lesion.
Authors: Bas E, Van De Water T, Lumbreras V, Rajguru S, Goss G, Hare J, Goldstein B
Stem Cells Dev, 2014;23(5):502-14.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Hypoxic conditioned medium from human amniotic fluid-derived mesenchymal stem cells accelerates skin wound healing through TGF-beta/SMAD2 and PI3K/Akt pathways.
Authors: Jun E, Zhang Q, Yoon B, Moon J, Lee G, Park G, Kang P, Lee J, Kim A, You S
Int J Mol Sci, 2014;15(1):605-28.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Inner ear hair cell-like cells from human embryonic stem cells.
Authors: Ronaghi, Mohammad, Nasr, Marjan, Ealy, Megan, Durruthy-Durruthy, Robert, Waldhaus, Joerg, Diaz, Giovanni, Joubert, Lydia-Ma, Oshima, Kazuo, Heller, Stefan
Stem Cells Dev, 2014;23(11):1275-84.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Specific ablation of Nampt in adult neural stem cells recapitulates their functional defects during aging.
Authors: Stein L, Imai S
EMBO J, 2014;33(12):1321-40.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Higher vulnerability and stress sensitivity of neuronal precursor cells carrying an alpha-synuclein gene triplication.
Authors: Flierl A, Oliveira L, Falomir-Lockhart L, Mak S, Hesley J, Soldner F, Arndt-Jovin D, Jaenisch R, Langston J, Jovin T, Schule B
PLoS ONE, 2014;9(11):e112413.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Reprogramming activity of NANOGP8, a NANOG family member widely expressed in cancer.
Authors: Palla, A R, Piazzolla, D, Abad, M, Li, H, Dominguez, O, Schonthaler, H B, Wagner, E F, Serrano, M
Oncogene, 2014;33(19):2513-9.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Sphingosine-1-phosphate promotes expansion of cancer stem cells via S1PR3 by a ligand-independent Notch activation.
Authors: Hirata, Naoya, Yamada, Shigeru, Shoda, Takuji, Kurihara, Masaaki, Sekino, Yuko, Kanda, Yasunari
Nat Commun, 2014;5(0):4806.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
FGFR2 is amplified in the NCI-H716 colorectal cancer cell line and is required for growth and survival.
Authors: Mathur, Anjili, Ware, Christop, Davis, Lenora, Gazdar, Adi, Pan, Bo-Sheng, Lutterbach, Bart
PLoS ONE, 2014;9(6):e98515.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Chondrogenesis of infrapatellar fat pad derived adipose stem cells in 3D printed chitosan scaffold.
Authors: Ye K, Felimban R, Traianedes K, Moulton S, Wallace G, Chung J, Quigley A, Choong P, Myers D
PLoS ONE, 2014;9(6):e99410.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Transcriptional profiling of adult neural stem-like cells from the human brain.
Authors: Sandberg C, Vik-Mo E, Behnan J, Helseth E, Langmoen I
PLoS ONE, 2014;9(12):e114739.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Human marrow stromal cells downsize the stem cell fraction of lung cancers by fibroblast growth factor 10.
Authors: Kanehira M, Kikuchi T, Santoso A, Tode N, Hirano T, Ohkouchi S, Tamada T, Sugiura H, Harigae H, Ichinose M
Mol Cell Biol, 2014;34(15):2848-56.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Genetic background drives transcriptional variation in human induced pluripotent stem cells.
Authors: Rouhani, Foad, Kumasaka, Natsuhik, de Brito, Miguel C, Bradley, Allan, Vallier, Ludovic, Gaffney, Daniel
PLoS Genet, 2014;10(6):e1004432.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Cis-acting DNA sequence at a replication origin promotes repeat expansion to fragile X full mutation.
Authors: Gerhardt J, Zaninovic N, Zhan Q, Madireddy A, Nolin S, Ersalesi N, Yan Z, Rosenwaks Z, Schildkraut C
J Cell Biol, 2014;206(5):599-607.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Generation of a vascularized and functional human liver from an iPSC-derived organ bud transplant.
Authors: Takebe, Takanori, Zhang, Ran-Ran, Koike, Hiroyuki, Kimura, Masaki, Yoshizawa, Emi, Enomura, Masahiro, Koike, Naoto, Sekine, Keisuke, Taniguchi, Hideki
Nat Protoc, 2014;9(2):396-409.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Histone hyperacetylation up-regulates protein kinase Cdelta in dopaminergic neurons to induce cell death: relevance to epigenetic mechanisms of neurodegeneration in Parkinson disease.
Authors: Jin H, Kanthasamy A, Harischandra D, Kondru N, Ghosh A, Panicker N, Anantharam V, Rana A, Kanthasamy A
J Biol Chem, 2014;289(50):34743-67.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Real-time discrimination between proliferation and neuronal and astroglial differentiation of human neural stem cells.
Authors: Lee, Rimi, Kim, Il-Sun, Han, Nalae, Yun, Seokhwan, Park, Kook In, Yoo, Kyung-Hw
Sci Rep, 2014;4(0):6319.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
CoCl2, a mimic of hypoxia, induces formation of polyploid giant cells with stem characteristics in colon cancer.
Authors: Lopez-Sanchez L, Jimenez C, Valverde A, Hernandez V, Penarando J, Martinez A, Lopez-Pedrera C, Munoz-Castaneda J, De la Haba-Rodriguez J, Aranda E, Rodriguez-Ariza A
PLoS ONE, 2014;9(6):e99143.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
MicroRNA screen of human embryonic stem cell differentiation reveals miR-105 as an enhancer of megakaryopoiesis from adult CD34+ cells.
Authors: Kamat V, Paluru P, Myint M, French D, Gadue P, Diamond S
Stem Cells, 2014;32(5):1337-46.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Tissue-engineered vascular grafts created from human induced pluripotent stem cells.
Authors: Sundaram S, One J, Siewert J, Teodosescu S, Zhao L, Dimitrievska S, Qian H, Huang A, Niklason L
Stem Cells Transl Med, 2014;3(12):1535-43.
Species: Human

内毒素水平
<0.10 EU per 1 μg of the protein by the LAL method.
生物活性
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.1-0.6 ng/mL.
The specific activity of Recombinant Human FGF basic is approximately 800 IU/μg, which is calibrated against recombinant human FGF basic WHO International Standard (NIBSC code: 90/712).
溶解方法
Reconstitute at 100-250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
背景

FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues, but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. A variety of forms of FGF basic are produced as a result of N-terminal extensions. These extensions affect localization of FGF basic in cellular compartments but do not affect biological activity. Binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to the same high affinity receptors and show a similar range of biological activities. FGF basic stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. FGF basic induces neuron differentiation, survival, and regeneration. FGF basic also modulates embryonic development and differentiation. These observed in vitro functions of FGF basic suggest FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may participate in the production of a variety of pathological conditions resulting from excessive cell proliferation and excessive angiogenesis.

SDS-PAGE
17 kDa, reducing conditions
存放说明
Frost Free Freezer -20 degrees C
纯度
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
不含载体
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

参考文献
  1. Coulier, F. et al. (1997) J. Mol. Evol. 44:43.
  2. Chen, C.H. et al. (2004) Curr. Vasc. Pharmacol. 2:33.
  3. Mohammadi, M. et al. (2005) Curr. Opin. Struct. Biol. 15:506.
  4. Fernig, D. et al. (1994) Prog. Growth Factor Res. 5:353.

参考图片

Recombinant Human FGF basic (146aa) (Catalog # 233-FB) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 0.1-0.6 ng/mL.

1 µg/lane of Recombinant Human FGF basic (146 aa) was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.

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